Which advantages do Eppendorf Polypropylene Microplates have over plates made from polystyrene?

Plates made from polypropylene may be used across a broad spectrum of applications, as they are more resistant to temperature variations as well as various chemicals than plates made from polystyrene. Furthermore, they are more robust, allowing them to be centrifuged at higher g-forces. In addition, biomolecules, such as nucleic acids and proteins, bind less strongly to polypropylene.

Are Eppendorf PP Microplates resistant to organic solvents, e.g. DMSO?

The Eppendorf PP Microplates are made from polypropylene and are thus resistant to DMSO and other organic solvents, unlike plates made from polystyrene. An overview of chemical resistance can be found in Application Note no. 56.

Are the colored rim and labeling resistant to organic solvents?

All areas of the plate, including the colored rim, are resistant to chemicals. The alpha numeric labeling is equally durable. An overview of chemical resistance can be found in Application Note no. 56.

How may the Eppendorf PP Microplates be sealed?

Eppendorf offers a variety of sealing options for Microplates. Self-adhesive sealers, films and foils for heat sealing, as well as re-usable sealing mats, are available. Information regarding the compatibility of the different sealing methods is listed in the Application Note no. 208.

Which advantages do the black Eppendorf PP Microplates offer?

Plates made from black material are especially well suited for fluorescence assays (e.g. PicoGreen ® ). The black wells prevent “cross talk” between wells and reduce background signals. The black Eppendorf PP Microplates feature the unique OptiTrack Matrix, the first technology to allow for fast and easy well identification in black plates. Due to carefully selected materials, the plates have very low autofluorescence. The superior signal-to-noise ratio contributes to the increased sensitivity of fluorescence assays (see Application Note no. 203).

Which advantages do the white Eppendorf PP Microplates offer?

Plates made from white material may be used for luminescence assays (chemiluminescence, scintillation proximity assays (SPA) etc.), as well as for fluorescence assays (fluorescence intensity, polarization, etc.). The white wells prevent “cross talk” between the wells while amplifying the individual signals. The white Eppendorf PP Microplates feature the unique OptiTrack Matrix, the first technology to allow for fast and easy well identification in white plates. Due to carefully selected materials, the plates offer maximum signal amplification. Due to their very low autofluorescence, the white Eppendorf Microplates are equally well suited for fluorescence assays. Taken together, these features allow for an improvement in the sensitivity of analyses.

May the Eppendorf PP Microplates be stored at –196°C in liquid nitrogen?

No, we recommend storage at a minimum of –80°C.

May the Eppendorf PP Microplates be autoclaved?

Yes; however, the exact specifications of the Microplates could be compromised. The plates are available in the qualities sterile or PCR clean. Hence, autoclaving is necessary only under exceptional circumstances.

Are the Eppendorf PP Microplates in LoBind quality coated or siliconized?

No, the LoBind quality of the Eppendorf PP Microplates is not derived from siliconization or any other type of coating. The functionality is achieved through a carefully selected material, in combination with a special manufacturing process.

How high may Eppendorf PP Microplates be centrifuged?

The Eppendorf PP Microplates may be centrifuged up to 6000 x g. Please follow the instructions provided in the manual.

May immunoassays such as ELISA be performed in the Eppendorf PP Microplates?

Plates made from polypropylene are not suited for the standard variant, where antigen or antibodies are bound to the well wall. For this application, plates made from polystyrene are used, which have a higher binding capacity for biomolecules. However, for antibody-based fluorescence or luminescence assays (e.g. with beads) Eppendorf PP Microplates with black or white wells are suitable, respectively.

Is it possible to perform absorption measurements in the Eppendorf PP Microplates?

Typically, absorption measurements are not performed in plates made from polypropylene, but in plates made from polystyrene, as this material generally has a very high transparency. Since the Eppendorf PP Microplates show considerably higher transparency compared to other plates made from polypropylene (see Application Note no. 202), the Eppendorf Microplates may be used for absorption measurements in certain cases.

Why are the Eppendorf PP Microplates offered in Sterile and PCR clean, but not in Eppendorf quality?

The Eppendorf PP Microplates are a very high quality product. In order to underline the quality, all plates are available in the degrees of purity PCR clean or Sterile. This increases the confidence and comfort of the user, who may employ the plates directly for valuable samples in sophisticated applications.

Are Eppendorf PP Microplates also available with barcode?

Yes, the option of Eppendorf Microplates with barcode is available. Further information can be found on the product page microplates .

Are Eppendorf products free of RNA?

All products certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: Where no human DNA is detected, there can also be no RNA, which is considerably more unstable.

What is the meaning of the PCR Clean seal?

For molecular-biological requirements Eppendorf offers a new standard for uniform purity specifications. All consumables marked with the "Certified PCR Clean” label are guaranteed free of human DNA, DNase, RNase and PCR inhibitors.

Why are there double labels on the folding boxes, as "PCR clean" + "Sterile" or "PCR clean" + "Protein-free"?

Due to the modularization of purity grades, some products are labeled with more than one purity grade symbol. For example, the epDualfilter T.I.P.S. has the double seal "PCR clean "+ "Sterile" and the UVette the double seal "PCR clean" + "Protein-free" on the packing. The certification remains unaffected by the modularization.

Are batch-specific certificates for the new purity grades available in the internet?

Yes, the batch-specific purity grades can be downloaded at our certificates homepage .

Can products of the previous and new modular purity grades (with new seals) be used simultaneously in one application?

Yes, if no batch attachment is required for the application, products of the old and new modular purity grades can be used, as their specifications are the same.

Are Eppendorf tubes and plates (also LoBind variants) resistant to 100% acetonitrile?

Eppendorf tubes and plates including LoBind variants are resistant against 100% acetonitrile only in room temperature conditions. Above 40°C and prolonged exposure (days) may lead to partial damage and is not recommended. For lower acetonitrile concentrations the resistance parameters will be better so prolonged exposure and higher temperatures may be possible. This must be however tested by the customer.

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Eppendorf Microplates

Eppendorf Microplate with OptiTrack® matrix

Sample being pipetted into a microplate with Combitip® pipette tips

Eppendorf Microplate in an automated liquid handling instrument

Four microplates, 384-well black and transparent plate, 96-well white and transparent plate

Selection of microplates stacked on top of each other

Woman in lab coat looking through microplate

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使用Eppendorf Microplates微孔板体验新水平的透光度！使用高品质聚丙烯和卓越的孔设计，您可以在移液，管理和回收样品时更省力。另外，我们提供适合每一种用途的平板。

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用途

特征

使用Eppendorf Microplates微孔板对您的样品轻松进行移液、管控和回收！采用易于识别的OptiTrack^®字母数字标签设计，可识别各个孔，提高加样速度，减少加液错误。Eppendorf Microplates微孔板由高透明度聚丙烯制成，可确保快速轻松地定位样品，如果您需要尽可能高的回收样品时可以选择Eppendorf LoBind^®。

卓越的透明度和OptiTrack^®Matrix

请放心，我们的Eppendorf Microplates微孔板采用优质聚丙烯制成，可确保卓越的透明度并提高结果再现性。高对比度OptiTrackMatrix有助于提高实验室效率——可快速识别孔井，并降低出错和污染风险。
了解关于Eppendorf Microplates微孔板如何用于快速鉴定样品的更多信息，请参阅应用文献第202期：Eppendorf聚丙烯微孔板 – 快速准确识别样品。

RecoverMax^®圆锥形孔设计可在短时间内实现更大回收率

Eppendorf微孔板采用RecoverMax^®优化的孔井结构，可实现较好的样品回收率和混匀性能。孔角的圆形平滑设计可以防止样品损失并尽可能减少残留，同时方孔工作板的光滑轮廓减少了交叉污染风险。

Eppendorf Microplates微孔板具备高透明度和品质，可提供各种型号：

• 496和384孔板
• F底（平底）、U型底（圆形底）和 V型底（锥形底）
• Eppendorf PCR clean洁净级和Sterile无菌级纯度级别
• 还提供DNA和Protein LoBind低吸附型号
• g–Safe^®高离心稳定性，可达 6.000 x g，可加速实验流程并保护样品质量。
• 凸起的孔缘和光滑的表面保证了密封性，即使是热密封。
• 优质的聚丙烯保证了对化学品、机械应力和极端温度的高耐性，同时还耐
• 高压灭菌（121 °C，20 分钟）。
• 生产过程不使用脱模剂、塑化剂或抑菌剂这些会对生物测定造成负面影响的材料。

详情请参阅应用文献第56期：适用于原装Eppendorf Tubes^®离心管和工作板的优质材料：聚丙烯特性及耐化学性。应用文献第 239 期：Eppendorf Deepwell Plates深孔板和微孔板 - 密封方法查询。