Eppendorf BioSpectrometer® basic

Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
Eppendorf BioSpectrometer® basic
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Eppendorf BioSpectrometer® basic 使您的工作变得更加轻松,具有以下特点: 操作简便,导航软件设计,直接在设备上操作,预设应用程序,测量数据自动换算,结果清晰。 更多信息

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产品信息

基于 Eppendorf BioPhotometer D30 版本的 Eppendorf BioSpectrometer basic 具备简便性、灵活性和可靠性。 除具备 Biophotometer D30 的所有特征外,Biospectrometer basic 可在 200  –  830 nm 范围内自由选择波长(1  nm 步进)。 这使用户可以自行定义单波长检测、多波长检测或光谱扫描的参数。 Eppendorf BioSpectrometer basic 不仅适合于预设的常规程序,还能编辑非预设或新的程序用于更多的应用。所以,The Eppendorf BioSpectrometer basic 是一款灵活而耐用的分光光度计。

Looking for the target?


The Eppendorf µCuvette is optimized for very low sample volumes. The hydrophobic surface coating on quartz glas enables convenient sample loading.

Eppendorf BioSpectrometer® basic

Left? Right? Up? Down?


Clear indications for positioning the cuvette for convenient handling and safe results.

Eppendorf BioSpectrometer® basic

Believing in raw data?


In general, the purity of a DNA solution is easily defined using purity ratios. It is possible that reduced purity may be attributed to the fact that the sample is solubilized in water instead of in buffer. Since water and buffer have different properties, the absorbance of the sample being measured will be affected by this disparity.
For this reason, the BioSpectrometer is capable of recording a “Purity Scan” which displays the absorbance properties of a sample graphically, thus enabling fast and simple visual quality control.

Eppendorf BioSpectrometer® basic

Looking for easy-to-read results?


In addition, all important raw data pertaining to the DNA sample are displayed in table format. The table optionally lists the purity ratios (A260/A230 and A260/A280), the absorbance values (raw data), as well as the background absorbance, e.g. at 320 nm.
Thus, the two display formats complement each other perfectly to allow for an evaluation of the quality of a DNA sample.

Eppendorf BioSpectrometer® basic

Annoyed by uncomfortable handling?


Ergonomics is far beyond the »ergonomically designed chair«. Eppendorf started to optimize the laboratory devices regarding ergonomics already in the early 1970s.
In 2003, we started the PhysioCare Concept, focusing on ergonomic liquid handling devices like our pipettes.
Nowadays, the Eppendorf PhysioCare Concept is broaden up to include further laboratory products as a holistic solution to harmonize the workflow in your laboratory with your health and well-being, e.g. for the Eppendorf BioSpectrometer:
› Guided software for easy handling
› Clear indications at cuvette shaft for convenient handling
› Direct keys for convenient handling
› Small footprint to enable position in direct arm reach at your bench
› Standardized »user-interface concept« for intuitive handling of devices

Further information about the Eppendorf PhysioCare Concept:
www.eppendorf.com/physiocare/

Eppendorf BioSpectrometer® basic

Struggling with paper-based documentation?


The eLABJournal Electronic Lab Notebook offers an intuitive and flexible solution to document research and track research data like BioSpectrometer–based values. Improve efficiency when documenting, organizing, searching, and archiving collected data. With the free eLABJournal add-ons, you can extend the functionality of eLABJournal to a fully integrated Laboratory Information Management System.

Start a free 30-day trial!
Visit www.eLABJournal.com

Eppendorf BioSpectrometer® basic

产品特性

  • 对单个波长或多个波长进行吸光度测定,波长扫描范围从 200  nm 到 830  nm(增量:1 nm)
  • 自动换算并储存测量数据,结果一目了然
  • 通过光谱图显示样品纯度,并自动进行比率值计算
  • 可自由编辑应用程序及可自由修改程序参数,通过因子、标准品或标准曲线进行浓度换算
  • 双波长法,带减法或除法换算
  • 集成应用和结果记忆功能
  • 通过 USB 接口、以太网、电子邮件的方式传输结果或直接打印输出结果
  • 导航软件设计,可减少误差
  • 集成自检和校准历史记录

应用

  • 核酸定量检测
  • 蛋白质直接定量检测 (UV 280 nm)
  • 通过 µCuvette G1.0 微量比色皿对高浓度样品进行微量检测
  • 细菌生长测定 (OD 600)
  • 通过比色法进行蛋白质定量测定,例如 BCA、Bradford、Lowry 法
  • 染料标记生物大分子(核酸或蛋白质)的结合率 (FOI) 评估
  • 可自由选择波长,例如,340 nm: NADPH 或 NAPH 测定,405 nm: 对硝基苯酚测定,420 nm: 邻硝基苯酚测定,490 nm: 比色法果糖测定,490 nm: 细胞毒性测定
 BioSpectrometer® basicBioSpectrometer® basic
BSA 浓度范围 (UV 280 nm) µCuvette® G1.0758 ng/µL -45,450 ng/µL
A = 0,±30 ng/µL
A = 1,±76 ng/µL (0.5 %)
BSA 浓度范围 (UV 280 nm) UVette® 10 mm75 ng/µL - 4,545 ng/µL
A = 0,±3 ng/µL
A = 1,±7 ng/µL (0.5 %)
BSA 浓度范围 (UV 280 nm) UVette® 2 mm379 ng/µL - 22,725 ng/µL
A = 0,±15 ng/µL
A = 1,±38 ng/µL (0.5 %)
BSA 浓度范围(适用 1 至 10 mm 光程长度)76 ng/µL - 45,450 ng/µL
(BSA 因数为 1,515)
dsDNA 浓度范围 (UV 260 nm) µCuvette® G1.025 ng/µL – 1,500 ng/µL
dsDNA 浓度范围 (UV 260 nm) UVette® 10 mm2.5 ng/µL – 150 ng/µL
dsDNA 浓度范围 (UV 260 nm) UVette® 2 mm12.5 ng/µL – 750 ng/µL
dsDNA 浓度范围(适用 1 至 10 mm 光程长度)2.5 ng/µL – 1,500 ng/µL
光源(吸收光)氙气闪光灯
光源(荧光)
光谱带宽≤4 nm
吸光度测量范围0 A – 3.0 A (260 nm)0 A – 3.0 A
存储方法>100 个方法程序
扫描
操作员指南语言西班牙语, 意大利语, 法语, 英语, 德语, 日语
最小步长1 nm
样品光程长度8.5 mm
检测器类型CMOS 二极管阵列,1024 像素
比色皿槽12.5 mm × 12.5 mm
波长范围200 nm – 830 nm
波长范围(吸收光)扫描波长 (nm): 200 – 830 nm,增量为 1 nm
波长选择增量1 nm
测量原理(吸收光)带参比光束的单光束原子吸收分光光度测定
测量原理(荧光)
温度系统误差
温度随机误差
系统误差(吸光度)±1 % (A = 1)
系统误差(波长)≤0.5 nm
能耗约 15 W,操作期间
约 5 W,显示屏变暗后
荧光发射波长 I
荧光发射波长 II
荧光检测范围
荧光检测随机误差
荧光激发波长
随机误差(吸光度)≤0.002,A = 0
≤0.005 (0.5 %),A = 1
≤0.002,A = 0
≤0.005 (0.5 %),A = 1
随机误差(波长)±1 nm
接口› USB 主接口: 用于连接 U 盘和热敏打印机 DPU-S445
› USB 设备接口用于连接计算机(即使没有计算机也可实现所有功能)
› 串行接口 RS 232: 用于连接热敏打印机 DPU-414
› 以太网接口 RJ45: 用于连接网络打印机或通过电子邮件直接从设备发送数据结果
电源230 V, 50 – 60 Hz230 V, 50 – 60 Hz
尺寸 (W × D × H)295 × 400 × 150 mm / 11.6 × 15.7 × 6 in
重量(不含附件)5.4 kg
两个测量点之间的间隔
测量时间范围
温控范围

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